| DNA与邻苯二胺-过氧化氢-过氧化物酶体系相互作用的光谱研究 |
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| 引用本文： | 曹瑛,何锡文. DNA与邻苯二胺-过氧化氢-过氧化物酶体系相互作用的光谱研究[J]. 分析化学, 1998, 26(10): 1165-1168 |
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| 作者姓名： | 曹瑛 何锡文 |
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| 作者单位： | 南开大学化学系!天津300071 |
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| 基金项目： | 国家自然科学基金，国家教委博士点基金 |
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| 摘 要： | 用光谱方法研究了邻苯二胺－过氧化氢－过氧化物酶体系与ＤＮＡ的作用，认为二者之间发生的是嵌插作用。分别用ＵＶ－Ｖｉｓ光谱和荧光光谱求得了形成常数，两种方法具有一致性。讨论了ｐＨ值对嵌插作用的影响。
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| 关 键 词： | 脱氧核糖核酸 邻苯二胺 辣根过氧化物酶 嵌插作用 |
Interaction Between Deoxyribonucleic Acid and o - Phenylenediamine - Hydrogen Peroxide - Rorseradish Peroxidease |
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| Cao Ying,He Xiwen. Interaction Between Deoxyribonucleic Acid and o - Phenylenediamine - Hydrogen Peroxide - Rorseradish Peroxidease[J]. Chinese Journal of Analytical Chemistry, 1998, 26(10): 1165-1168 |
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| Authors: | Cao Ying He Xiwen |
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| Abstract: | The interaction between o - phenylenediamine - hydrogen peroxide - horseradish peroxidease (OPD-H2O2-HRP) and deoxyribonucleic acid(DNA) was studied by spectroscopic method. It was proposed that there was an intercalation between them, and the 2, 3-diaminophenazine (DAP), product of OPD oxided by H2O2 catalyzed by HRP, acts as intercalator. The formation constant between OPD- H2O2HRP and DNA was obtained by ultraviolet-visible and fluoresoence spectra. Effect of pH value on intercalation was also studied. |
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| Keywords: | Deoxyribonucleic acid o-phenylenediamine horseradish peroxidease intercalation |
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