傅里叶变换红外光谱实时监测乳腺癌细胞化疗反应性的研究

应用傅里叶变换红外光谱技术监测乳腺癌细胞株MCF-7在化疗药物5氟尿嘧啶(5-FU)干预过程中相应的红外光谱变化，验证其与药物作用的时间和剂量相关性。对数据结果整理发现，在时间相关性方面，随药物作用时间的延长，乳腺癌细胞株MCF-7对脂类的利用率下降，细胞中脂类含量增加，同时肿瘤细胞分裂增殖受阻，细胞内DNA和RNA的含量降低，实验中观察到，48 h内，乳腺癌细胞MCF-7的红外光谱中代表脂类变化的峰强比I_{2 920}/I_{1 460}逐渐升高，I_{1 400}/I_{1 460}逐渐降低，代表细胞内核酸变化的峰强比I_{1 080}/I_{1 550}，I_{1 240}/I_{1 550}逐渐降低；在剂量相关性方面，癌细胞红外光谱中峰强比I_{1 640}/I_{1 550}较正常升高，实验中观察到峰强比I_{1 640}/I_{1 550}随药物浓度的增加而呈现逐渐降低的趋势。以上这些变化与乳腺癌细胞在化疗药物干预过程中的生物学改变相符，可作为FTIR实时监测乳腺癌细胞化疗反应性的参考指标。

Application of FTIR Spectrometry to Monitoring Chemosensitivity of Breast Cancer Cell Line Using 5-FU

The purpose of the present study was to compare the changes of infrared spectrum of breast cancer cell line MCF-7 treated by 5-fluorouracil with various concentrations and treating time. The results indicate that the relative intensity ratios of I_{2 920}/I_{1 460} increased in the FTIR spectra of cell line MCF-7 while I_{1 400}/I_{1 460}, I_{1 080}/I_{1 550} and I_{1 240}/I_{1 550} decreased significantly within forty-eight hours, however, no regular change was observed during 48～72 hours. The relative intensity ratio of I_{1 640}/I_{1 550} decreases along with the increase in the concentration of 5-fluorouracil. The changes of infrared spectra are consistent with the biochemical changes in breast cancer cell, and it can be the reference designators when assessing curative effect of breast cancer cell by FTIR spectrometry.