恩诺沙星分子印迹纳米纤维膜的制备及性能

通过沉淀聚合法制备了恩诺沙星（ENRO）分子印迹聚合物（MIPs）微球，将其添加到聚乙烯醇（PVA）溶液中，采用静电纺丝技术制备了恩诺沙星分子印迹纳米纤维膜（MINFMs）. 利用扫描电子显微镜（SEM）研究了纺丝液浓度、纺丝电压及接收距离对MINFMs纤维直径及表面形貌的影响，从溶胀性、孔隙率、吸附容量及吸附选择性等几个方面对印迹膜的性能进行了评价. 结果表明，在环境温度25 ℃、相对湿度 40%~50%、MIPs加入量8%（质量分数）、PVA质量分数7%、纺丝电压15 kV和接收距离25 cm的条件下，得到的MINFMs的纤维形态良好，纤维平均直径为180 nm. MINFMs的溶胀度和孔隙率分别为136.76%和33.42%，均大于非印迹纳米纤维膜（NINFMs）. 动力学吸附性能结果显示，MINFMs在300 min后吸附基本达到平衡，且明显高于NINFMs的吸附量；Scatchard分析结果表明，在所研究的浓度范围内MINFMs对模板ENRO的结合位点是等价的，其离解平衡常数（K_d）与最大表观结合量（Q_max）分别为505.817 mg/L和3.862 mg/g. 与环丙沙星（CIP）和氧氟沙星（OFL）相比，MINFMs对ENRO表现出更强的特异性吸附能力.

Preparation and Properties of Molecularly Imprinted Nanofiber Memberanes Towards Enrofloxacin

Enrofloxacin(ENRO) molecular imprinted polymers(MIPs) obtained via precipitation polymerization were added to the polyvinyl alcohol(PVA) aqueous solution. Then electrospinning technique was employed to prepare ENRO molecularly imprinted nanofiber membranes(MINFMs). We investigated the effects of concentration of PVA solution, applied voltage, and the distance between the needle and collector on the diameter and morphology of the nanofiber membrane via scanning electron microscope(SEM) measurement. The swelling ratio, void ratio, adsorption property, and adsorption selectivity of membrane were also studied. The optimal electrospinning process was carried out at environmental temperature of 25 ℃ and relative humidity of 40%—50%, with PVA concentration of 7.0%(mass fraction), spinning voltage of 15 kV, and the distance between the needle and collector of 25 cm. Under such conditions, the fibers in MINFMs were uniform with diameters of about 180 nm. The swelling rate and void ratio of the as-prepared MINFMs were 136.76% and 33.42%, respectively, which were more than those of non-imprinted nanofiber membranes(NINFMs). The dynamic adsorption experiments of MINFMs showed that the adsorption reaction reached balance after 300 min. Analysis of the Scatchard plot revealed that the binding sites of MINFMs to ENRO were equal class under the studied concentration range, the dissociation constant(K_d) and apparent maximum adsorption quantity(Q_max) of MINFMs were 505.817 mg/L and 3.862 mg/g, respectively. The specific absorption capacity of MINFMs to ENRO was higher than those of ciprofloxacin(CIP) and ofloxacin(OFL).