Tandem Time-of-Flight Mass Spectrometer with High Precursor Ion Selectivity Employing Spiral Ion Trajectory and Improved Offset Parabolic Reflectron |
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Authors: | Takaya Satoh Takafumi Sato Ayumi Kubo Jun Tamura |
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Affiliation: | (1) JEOL Ltd., 3-1-2 Musashino, Akishima 196-8558, Tokyo, Japan |
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Abstract: | In this study, we have developed a tandem time-of-flight mass spectrometry (TOF/TOF) technique involving the use of a matrix-assisted
laser desorption/ionization ion source that exhibits high precursor ion selectivity. An ion optical system with a 17 m spiral
ion trajectory was used in the first time-of-flight mass spectrometer. High precursor ion selectivity was achieved by realizing
a 15 m flight path, which is considerably longer than that of the conventional MALDI-TOF/TOF before the precursor ion selection
by an ion gate; monoisotopic ions could be selected properly up to m/z 2500. Furthermore, the first time-of-flight mass spectrometer was composed of electrostatic sectors and could eliminate post-source
decay (PSD) ions. Precursor ions with 20 keV kinetic energy were selected and injected into a collision cell, leading to the
generation of fragment ions by high-energy collision-induced dissociation (HE-CID). The optimized second time-of-flight mass
spectrometer included a post-acceleration region and an offset parabolic reflectron to record product ion spectra in the entire
mass range. Our system could generate a simple HE-CID product ion spectrum because each fragment pathway could be observed
as a single peak by the selection of monoisotopic ions of all precursor ions and HE-CID fragment pathways could be predominantly
observed by the PSD ion elimination. |
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