| 化学发光法测定痕量绿原酸 |
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| 引用本文： | 周娟,陈荟芸,杨华松,徐明波,吕鉴泉. 化学发光法测定痕量绿原酸[J]. 分析试验室, 2012, 0(2): 13-16 |
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| 作者姓名： | 周娟 陈荟芸 杨华松 徐明波 吕鉴泉 |
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| 作者单位： | 湖北师范学院化学与环境工程学院;黄石市中心血站 |
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| 基金项目： | 湖北省自然科学基金(2010CDA061);湖北省教育厅中青年项目(Q20112507);湖北师范学院研究生科研启动基金(2008D54)资助 |
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| 摘 要： | 基于绿原酸对鲁米诺-H2O2-辣根过氧化物酶化学发光体系具有强烈的抑制作用,建立了绿原酸的化学发光分析法,并探讨了其作用机理。化学发光强度的变化值与绿原酸浓度的对数在5.2×10-9～1.0×10-6g/mL范围内呈线性关系,检出限(S/N=3)达7.8×10-10g/mL。该方法成功用于金银花中绿原酸含量的测定,回收率为96.0%～100.7%。
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| 关 键 词： | 化学发光 鲁米诺 绿原酸 辣根过氧化物酶 |
Determination of trace chlorogenic acid by chemiluminescence method |
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| Affiliation: | ZHOU Juan1,CHEN Hui-yun1,YANG Hua-song2,XU Ming-bo1 and LU Jian-quan1(1.College of Chemistry and Environmental Engineering,Hubei Normal University,Huangshi 435002;2.Huangshi Blood Station,Huangshi 435002) |
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| Abstract: | Based on the inhibition of chlorogenic acid on the chemiluminescence reaction of luminol-H2O2-horseradish peroxidase,a new chemiluminescence method has been developed for the determination of trace chlorogenic acid.The method was convenient,rapid and sensitive.The change of chemiluminescence intensity(ΔI) had a linear relationship with the logarithm of chlorogenic acid concentration in the range of 5.2×10-9～1.0×10-6 g/mL,and the detection limit(S/N=3) was 7.8×10-10 g/mL.The method has been successfully applied to the determination of chlorogenic acid in Lonocera Japonica Thunb samples,and the recoveries were 96.0%～100.7 %.Futhermore,the inhibition mechanism was discussed in this paper. |
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| Keywords: | Chemiluminescence Luminol Chlorogenic Acid Horseradish Peroxidase |
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