Thermal Stability of Haemoglobin Solutions Under DC and AC Magnetic Field and UV and IR Radiation

The influence of electromagnetic field in wide range of frequency (extra low frequency magnetic field, monochromatic laser light 840 nm, ultraviolet light) and static magnetic field on the stability of aqueous and NaCl 0.9% haemoglobin solutions was studied using differential scanning calorimetry (DSC) method. Three steps of denaturation process can be derived from experimental data. The shifts of transition temperatures are more marked for NaCl 0.9% than for aqueous solutions. The results suggest that static magnetic field is effective in protecting protein, while ultraviolet radiation destabilises haemoglobin. The laser light and alternating magnetic field have a little effect on the thermal stability of haemoglobin. This revised version was published online in July 2006 with corrections to the Cover Date.