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Attachment of tyrosinase on mixed self-assembled monolayers for the construction of electrochemical biosensor |
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| Authors: | Xue Ping Ji Xian Rui Li Na Wang Rui Xing Ni Xiao Hong Liu Hua Ai Xiong |
| Institution: | Department of Medical Chemistry, Hebei Medical University, Shijiazhuang 050017, China |
| Abstract: | A mixed self-assembled monolayers (SAMs) of thioctic acid (T-COOH) and thioctic acid amide (T-NH2) was used to immobilize tyrosinase for fabricating biosensor. The results showed that the mixed SAMs prepared from solution at the ratio of 1:4 provided an excellent microenvironment for enzymatic reaction between tyrosinase and substrate. The biosensor exhibited a fast response and high sensitivity for sensing substrate. |
| Keywords: | Biosensor Tyrosinase Catechol Self-assembled monolayers Gold electrode |
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