| 碳纤维超微盘电极的制备及对单囊泡胞吐动力学的高分辨研究 |
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| 引用本文： | 李雷,李照玉,万敏,张蓉颖.碳纤维超微盘电极的制备及对单囊泡胞吐动力学的高分辨研究[J].分析科学学报,2009,25(4). |
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| 作者姓名： | 李雷 李照玉 万敏 张蓉颖 |
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| 作者单位： | 分子生物物理教育部重点实验室,华中科技大学生命科学与技术学院,湖北武汉,430074 |
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| 基金项目： | 国家自然科学基金，科技部国家重大科学研究计划蛋白质专项，湖北省自然科学基金 |
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| 摘 要： | 利用改良的电化学腐蚀方法制备了碳纤维超微盘状电极,具有方法简便,电极尺寸可控、可多次使用等优点.扫描电镜和伏安表征实验表明电极密封效果良好、电化学性能优良.在鼠嗜铬神经瘤PC12细胞上,用超微盘电极实现了高时空分辨、高保真度的单囊泡胞吐测定.与常规微电极相比,能更准确地反应囊泡融合这一超快过程(毫秒级)的动力学过程.
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| 关 键 词： | 超微盘电极 电化学检测 囊泡 胞吐 融合动力学 |
Fabrication of Ultra-micro Disk Carbon Fiber Electrode that Enables Monitoring Single Vesicle Release Kinetics with High Sensitivity |
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| LI Lei,LI Zhao-yu,WAN Min,ZHANG Rong-ying.Fabrication of Ultra-micro Disk Carbon Fiber Electrode that Enables Monitoring Single Vesicle Release Kinetics with High Sensitivity[J].Journal of Analytical Science,2009,25(4). |
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| Authors: | LI Lei LI Zhao-yu WAN Min ZHANG Rong-ying |
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| Institution: | LI Lei,LI Zhao-yu,WAN Min,ZHANG Rong-ying(Key Laboratory of Molecular Biophysics of the Ministry of Education,College of LifeScience and Technology,Huazhong University of Science and Technology,Wuhan 430074) |
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| Abstract: | Carbon ultramicroelectrode has attractive potential application in probing neurotransmitter release at synapse terminals and fusion pore dynamics.In this study,we reported a facile method of fabricating ultra-micro disk carbon fiber electrodes(CFEs) using a refined electrochemical etching and plastic insulation techniques.The fabricated disk electrodes show ideal steady-state voltammetric behaviors,and SEM micrographs show the tips are fine ultra-micro disk,while the geometry of electrodes is fairly uniform... |
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| Keywords: | Ultra-micro disk electrode Electrochemical detection Vesicle Exocytosis Fusion pore dynamics |
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