激光照射血细胞的衰变规律和损伤机制研究

采用FLS900型稳态荧光光谱仪对红色激光持续照射的正常离体血液和酒精中毒离体血液进行了荧光检测,得到了血液物质成分变化及荧光光谱特征随时间衰变的规律.研究结果表明：随着血液离体时间的延长,当407 nm的紫光激励时,在605 nm处的荧光峰峰位保持不变只是强度缓慢减弱,但两者均在离体后的14 h和10 h出现了谱形突变,并在474 nm处产生了一个强荧光峰.比较分析发现激光对正常离体血液的衰变有明显的延缓作用,对酒精作用破坏的红细胞也有部分修复功能,但当激光持续照射一定时间后却导致了细胞的形态改变进而加速了细胞的溶血进程,产生了大量的内源性荧光物质还原烟碱腺嘌呤二核苷酸.本文的研究结果能为血细胞活性衰变规律以及功能结构研究提供有意义的参考.

Decay and Damage Mechanism of Blood Irradiated by Laser

The FLS900 Steady-state fluorescence spectrometer is used to test the normal and alcohol blood in vitro when radiated by continuous laser,and the variation components in blood and the fluorescence characteristics decayed with the time are obtained.The results show that the location of the fluorescence peak can not change when excited by 407 nm laser,only the intensity of the emission peak in the 605 nm decreases slowly.But the fluorescence spectrums have a wave mutation in 14 hours and 10 hours respectively,and have a strong fluorescence peak in the 474 nm eventually.Through the analysis,it can be concluded that the laser can put off the decay time of blood in vitro obviously and also can partly repaired the erythrocyte destroyed by the alcohol,but continuous irradiation by laser led to the change of cells morphology and then accelerated the hemolytic process,thus produced a lot of fluorescence material-reduction of nicotinamide adenine dinucleotide.The result will supply a valuable reference to the research on activity of the blood cell and the functional structure of the blood.