基于荧光光谱技术研究食用豆油的生物效应

采用FLS900荧光光谱仪对食用豆油的老鼠全血溶液与正常全血溶液进行了荧光光谱对比分析,得到了食用豆油以及正常老鼠血液的光谱特征差异.实验结果表明,当采用407 nm的激光激励全血溶液时,会发出峰值分别位于515 nm、556 nm和610 nm处的荧光.食用豆油的血液荧光强度比正常血液的荧光强度小,其荧光偏振度也小于正常血液的偏振度.分析认为长期食用豆油导致血细胞表面积变小,即发光面积变小,引起荧光强度减小;体积变小,使得分子的旋转能力增强,发射荧光记忆入射光的能力减弱,去偏效果明显增强,因而荧光偏振度变小.研究结果表明,长期食用豆油后能有效使人体血液粘度减小,改善血液循环,为食用油的营养价值分析及指导人们正确食用油脂提供参考.

Biological Effects of Eat Soybean Oil Based on the Fluorescence Spectroscopy

A comparative analysis of fluorescence spectra of whole blood solution in mice having soybean oil and that in normal mice is conducted through the FLS900 steady-state fluorescence spectrometer. The results show that when using 407 nm laser to stimulate whole blood solution, it will be issued the fluorescence with peaks at 515 nm, 556 nm and 610 nm. The fluorescence intensity of having soybean oil blood less than that of normal blood. Fluorescence polarization is also less than normal polarization of blood. The analysis shows that long-term consumption of soybean oil leads to blood cell surface area smaller that light-emitting area smaller, causing the fluorescence intensity decreases. The volume becomes smaller and enhances the ability of molecular rotation. The ability of launch fluorescence memories' incident light is decreased and remove polarization effect is obvious enhanced so that fluorescence polarization becomes smaller. The results of the study show that long-term having soybean oil can effectively make human body blood viscosity decrease and improve blood circulation, which provide a reference for people having edible oils correctly.