SIGNIFICANCE OF FLUENCE-RESPONSE DATA IN PHYTOCHROME-INITIATED SEED GERMINATION

Abstract. A model was developed to describe changes in fluence-response kinetics in terms of total phytochrome level (P_tot), level of the factor X interacting with active phytochrome (P_fr), P_frX equilibrium constant, seed sensitivity to P_fr-X interaction, and variation in phytochrome sensitivity within the seed population. Under conditions of stable X levels and stable population variation, the model predicted that a change in any of the other components will result in a parallel fluence-response curve on a probit-logarithmic plot. The linearity of the subsaturation plot is dependent on the ratio of P_tot to X concentrations. The model showed that changes in threshold response fluences can result from many causes other than changes in total phytochrome P_tot>]. Changes in response-saturating fluences when maximal germination is less than 100% are predicted to be due to limiting levels of X. Changes in slope of fluence-response curves can be explained by changes in seed population variation by this model. Rumex crispus L. fluence-response data for germination is best explained by this model in terms of neither changes in P_tot nor X levels altering kinetics.