A “Distorted‐BODIPY”‐Based Fluorescent Probe for Imaging of Cellular Viscosity in Live Cells |
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Authors: | Hao Zhu Dr. Jiangli Fan Miao Li Jianfang Cao Dr. Jingyun Wang Prof. Dr. Xiaojun Peng |
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Affiliation: | 1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High‐tech District, Dalian 116024 (China), Fax: (+86)?411‐84986306;2. Department School of Life Science and Biotechnology, Dalian University of Technology, No. 2 Linggong Road, High‐tech District, Dalian 116024 (China) |
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Abstract: | Cellular viscosity is a critical factor in governing diffusion‐mediated cellular processes and is linked to a number of diseases and pathologies. Fluorescent molecular rotors (FMRs) have recently been developed to determine viscosity in solutions or biological fluid. Herein, we report a “distorted‐BODIPY”‐based probe BV‐1 for cellular viscosity, which is different from the conventional “pure rotors”. In BV‐1 , the internal steric hindrance between the meso‐CHO group and the 1,7‐dimethyl group forced the boron–dipyrrin framework to be distorted, which mainly caused nonradiative deactivation in low‐viscosity environment. BV‐1 gave high sensitivity (x=0.62) together with stringent selectivity to viscosity, thus enabling viscosity mapping in live cells. Significantly, the increase of cytoplasmic viscosity during apoptosis was observed by BV‐1 in real time. |
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Keywords: | dyes fluorescent probes imaging agents steric hindrance viscosity |
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