NAD(P)H‐Independent Asymmetric CC Bond Reduction Catalyzed by Ene Reductases by Using Artificial Co‐substrates as the Hydrogen Donor |
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| Authors: | Dr. Christoph K. Winkler Dorina Clay Marcello Entner Markus Plank Prof. Dr. Kurt Faber |
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| Affiliation: | Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz (Austria), Fax: (+43)?316‐380‐9840 |
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| Abstract: | To develop a nicotinamide‐independent single flavoenzyme system for the asymmetric bioreduction of C?C bonds, four types of hydrogen donor, encompassing more than 50 candidates, were investigated. Six highly potent, cheap, and commercially available co‐substrates were identified that (under the optimized conditions) resulted in conversions and enantioselectivities comparable with, or even superior to, those obtained with traditional two‐enzyme nicotinamide adenine dinucleotide phosphate (NAD(P)H)‐recycling systems. |
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| Keywords: | alkene reduction artificial biocatalysis ene reductases enzyme catalysis hydrogen donors |
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