| 亲水型高分子包敷大孔硅胶基质的弱阳离子交换填料用于蛋白质分离 |
| |
| 引用本文： | 吴晓军,杨睦.亲水型高分子包敷大孔硅胶基质的弱阳离子交换填料用于蛋白质分离[J].分析化学,1997,25(1):52-55. |
| |
| 作者姓名： | 吴晓军 杨睦 |
| |
| 作者单位： | 中国科学院化学研究所!北京100080 |
| |
| 基金项目： | 国家科委863资助项目。 |
| |
| 摘 要： | 报道用亲水型高分子包敷大孔硅在质的羧甲基型（ＣＭ）高效弱阳离子交换色谱分离蛋白质的工作。经ＣＭ柱纯化的溶菌酶比活性提高７倍，活性回收率高于９５％，ＣＭ填料对溶蓖酶的容量达８０ｍｇ／ｇ。
|
| 关 键 词： | 液相色谱 弱阳离子交换剂 包敷硅胶 蛋白质 分离 |
Protein Separation By Weak Cation Exchange Chromatography With Hydrophilic Polymer-Coated Macroporous Silica Gel |
| |
| Wu Xiaojun,Yang Mu,Li Xingfa,Liu Guoquan.Protein Separation By Weak Cation Exchange Chromatography With Hydrophilic Polymer-Coated Macroporous Silica Gel[J].Chinese Journal of Analytical Chemistry,1997,25(1):52-55. |
| |
| Authors: | Wu Xiaojun Yang Mu Li Xingfa Liu Guoquan |
| |
| Abstract: | Carboxymethyl weak cation exchanger based on macroporous silica gel coated with hydrophilic polymer was synthesized for the separation of proteins. After purification with the column the specific activity of lysozyme was raised for 7 times with a >95% recovery. |
| |
| Keywords: | High performance liquid chromatography carboxymethyl weak cation exchang- er polymer coated silica gel protein |
| 本文献已被 CNKI 维普 等数据库收录！ |
