Qualitative and Semi-quantitative Analysis of Quaternary Alkaloids in Coptis-scute Herb Couple by Electrospray Mass Spectrometry |
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| Authors: | LI Wei SONG Feng-rui LIU Zhi-qiang LIU Shu-ying |
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| Institution: | [1]Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun Center of Mass Spectrometry, Changchun 130022, P. R. China [2]Graduate School of the Chinese Academy of Sciences, Beijing 100039, R R. China |
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| Abstract: | A practical solution of qualitatively analyzing quaternary alkaloids in coptis-scute herb couple by electrospray ionization mass spectrometry(ESI-MS)Was developed.Without the complicated pretreatment of sample,the active ingredients including berberine, palmatine, coptisine, jatrorrhizine, epiberberine, and columbamine were identified and some relative content changing rules of alkaloids in coptis-scute couple were summarized in this article. The overall profiles of the complex extracts were obtained.After adding an internal standard(rutaecarpine), semi-quantitative analysis was performed and the result indicates that the actual content of alkaloids was decreased by increasing the amount of scute.Based on the data obtained by high-performance capillary electrophoresis(HPCE),the feasibility of semi-quantitative analysis by ESI-MS WaS further proved. |
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| Keywords: | ESI-MS Coptis Scute Quaternary alkaloids |
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