| 共振光散射成像检测5,10,15,20-四(对三甲基氨基苯)卟吩诱导的DNA超螺旋聚集体 |
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| 引用本文： | 刘希东,黄承志,郭宏平,黄玉明. 共振光散射成像检测5,10,15,20-四(对三甲基氨基苯)卟吩诱导的DNA超螺旋聚集体[J]. 中国化学, 2006, 24(1): 89-94. DOI: 10.1002/cjoc.200690027 |
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| 作者姓名： | 刘希东 黄承志 郭宏平 黄玉明 |
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| 作者单位： | [1]College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China [2]Department of Chemistry and Environmental Sciences, Chongqing University of Arts and Sciences, Chongqing 402168, China |
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| 基金项目： | Project supported by the National Science Foundation for Preeminence Youth in China （No. 20425517）, the National Natural Science Foundation of China （No. 20275032）, the Scientific Research Fund of Chongqing Municipal Education Commission, Chunhui Program directed under the Ministry of Education of China, and the Municipal Science and Technology Committee of Chongqing. |
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| 摘 要： | A resonance light scattering （RLS） imaging method was proposed based on imaging and measuring the RLS features of single suprahelical species of DNA, and its appfication to DNA assay was also investigated. In acidic medium, porphine-5,10,15,20-tetrakis（p-phenyltlimethylaminium） （PTPTMA）, could stack along the molecular surface of DNA with the mode of long-range assembly to induce the formation of suprahelical species of DNA, resulting in strong RLS signals in the range of 450-510 nm. Under the excitation of 488 nm fight beam of argon ion laser source, single suprahelical species could be observed with the aid of a common microscope due to the strong scattered fight emitted by the suprahelical species. By capturing the RLS images of the single suprahelical species with a cooled charge coupled device （CCD） camera, and analyzing the RLS data, herein an RLS imaging method of DNA was proposed based on the linear relationship between the counts of suprahelical species in the detection focus plane and the concentration of DNA in nanograms. When 1.8 μmol/L PTPTMA was employed, both calf thymus DNA （ct DNA） and fish sperm DNA （fs DNA） in the range of 25-1100 ng/mL could be detected with the limits of detection lower than 25 ng/mL （3a）. Four synthetic samples were detected satisfactorily with relative standard deviations less than 5.1%.
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| 关 键 词： | 谐振光散射法 分光荧光计 DNA RLS技术 光束 分析化学 |
| 收稿时间： | 2004-11-16 |
| 修稿时间： | 2004-11-162005-03-17 |
Resonance Light Scattering Imaging Detection of Single Suprahelical Species of DNA Induced by Porphine‐5,10,15,20‐tetrakis(p‐phenyltrimethylaminium) |
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| Liu XiDong;Huang ChengZhi;Guo HongPing;Huang YuMing. Resonance Light Scattering Imaging Detection of Single Suprahelical Species of DNA Induced by Porphine‐5,10,15,20‐tetrakis(p‐phenyltrimethylaminium)[J]. Chinese Journal of Chemistry, 2006, 24(1): 89-94. DOI: 10.1002/cjoc.200690027 |
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| Authors: | Liu XiDong Huang ChengZhi Guo HongPing Huang YuMing |
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| Abstract: | |
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| Keywords: | porphine‐5,10,15,20‐tetrakis(p‐phenyltrimethylaminium) DNA resonance light scattering imaging |
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