Chiral discrimination ofN-(dansyl)-dl-amino acids on human serum albumin stationary phase: Effect of a mobile phase modifier |
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Authors: | E. Peyrin Y. C. Guillaume |
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Affiliation: | (1) Faculté de médecine et pharmacie, Laboratoire de chimie analytique, Place Saint Jacques, 25030 Besancon Cedex, France |
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Abstract: | Summary The effect of perchlorate anion as mobile phase modifier on the separation factor, α, forN-(dansyl)-dl-norvaline andN-(dansyl)-dl-tryptophan on a human serum albumin (HSA) column was studied by varying the concentration,c, of the chaotropic agent and the column temperatureT. Gibbs-Helmholtz parameters Δ(ΔH) and Δ(ΔS) between thed andl enantiomers were determined from linear van't Hoff plots of lnα against 1/T. Thermodynamic results indicated that the enhancement of the separation factor observed asc was increased was enthalpically controlled owing to stereoselective H-bonding interactions. Such behavior was used to optimize the chromatographic conditions for separation ofN-(dansyl)-amino acids on HSA. |
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Keywords: | Column liquid chromatography N-(dansyl)- font-variant:small-caps" >dl-amino acids Human serum albumin Chiral discrimination Optimization |
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