| 鲱精DNA纤维水合状态的拉曼分析 |
| |
| 引用本文： | 余多慰,柯惟中.鲱精DNA纤维水合状态的拉曼分析[J].光散射学报,1997,9(1):1-6. |
| |
| 作者姓名： | 余多慰 柯惟中 |
| |
| 作者单位： | [1]南京师范大学生物系 [2]南京师范大学理化测试中心 |
| |
| 基金项目： | 江苏省科委自然科学基金 |
| |
| 摘 要： | 本文对鲱精ＤＮＡ纤维２９００～３４００ｃｍ－１区间的拉曼光谱特征模进行分析，发现反映主链骨架Ｃ－Ｈ振动的２９６４和２９５０ｃｍ－１模对ＤＮＡ纤维空间构象有敏感性。在不同水合状态下，结合水Ｏ－Ｈ伸缩振动３２０４ｃｍ－１模比３２２０ｃｍ－１相对稳定，是计算纤维水合参数的重要依据。用公式２．５９２６×Ｉ２９６４／Ｉ３２０４求出的结合水分子的数量及样品相对湿度值，与Ｐｒｅｓｃｏｔ的数据相比，可见脱氧胸腺嘧啶核苷３１２９ｃｍ－１特征模所反映的Ｃ６－Ｈ振动强度与样品相对湿度值存在相关性
|
| 关 键 词： | 鲱精脱氧核糖核酸 拉曼光谱 水合 |
Raman Spectroscopic Investigation of the Hydration State of Herring Sperm DNA Fiber |
| |
| Yu Duowei,Ke Weizhong ,Zhang Xiran.Raman Spectroscopic Investigation of the Hydration State of Herring Sperm DNA Fiber[J].Chinese Journal of Light Scattering,1997,9(1):1-6. |
| |
| Authors: | Yu Duowei Ke Weizhong Zhang Xiran |
| |
| Institution: | Yu Duowei,Ke Weizhong **,Zhang Xiran |
| |
| Abstract: | Raman spectra in the 2900 3400cm -1 interval have been used to investigate molecular hydration state of herring sperm DNA fibers. We found that both features at 2964 and 2950 cm -1 assigned to main chain backbone C H vibration are sensitive to spatial conformation and the characteristic peak at 3204cm -1 is more stable than the peak at 3220cm -1 which is assiged to bound water O H stretching vibration. These two features form an important foundation to determine the hydration parameters for various fibers. Using the formula 2.5926×I2964/I3204, the value of bound water content and sample relative humidity (R.H.) have been calculated. Comparison with Prescoot’s results shows that there is correlation between the intensity of the 3129 cm -1 peak for thymidine C6 H vibration and the R.H.value of various fibers. |
| |
| Keywords: | herring sperm DNA Raman spectroscopy hydration |
| 本文献已被 CNKI 维普 等数据库收录！ |
