光交联技术的生物应用研究进展

光交联反应作为一种快速、简单和时空可控的交联工具广泛地应用于化学、生物、医学和材料等不同研究领域。本文详细介绍了常用的小分子光交联基团的结构、分类及反应机理,重点综述了光交联技术在生物医学领域的应用研究,并对其应用前景进行了展望。目前大多光交联基团仅对紫外和可见光具有敏感性,紫外和可见光穿透力弱、组织吸收强和散射等问题严重限制了该技术在生物体内的应用研究。因此,进一步研究光交联技术在生物体系的应用和开发长波长光(如近红外或远红外光)介导的新交联技术对于药物研发和疾病诊疗具有重要的科学意义。

Advances in biological application of photo-crosslinking technique

Photo-crosslinking technique is widely used in different research fields such as chemistry, biology, medicine and materials as a fast, simple and space-time controlled cross-linking tool. In this paper, the structure, classification and reaction mechanism of commonly used small-molecule photo-crosslinking groups are introduced in detail. The application of photo-crosslinking technique in biomedical fields is reviewed in detail, and the prospects for its application are assessed. Currently, most of the photo-crosslinking groups are only sensitive to ultraviolet and visible light, and have weak UV and visible light penetrating power, strong tissue absorption and scattering, which seriously limit the application of this technology in living system. Therefore, further research on the application of photo-crosslinking technology in biological systems and the development of new long-wavelength light-mediated crosslinking (such as near-infrared or far-infrared light) have important scientific significance for drug development and disease theranostics.