| 聚合酶键式反应生物芯片/微装置的实时定量荧光检测 |
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| 引用本文： | 章春笋,徐进良.聚合酶键式反应生物芯片/微装置的实时定量荧光检测[J].分析科学学报,2006,22(1):102-107. |
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| 作者姓名： | 章春笋 徐进良 |
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| 作者单位： | 1. 中国科学院广州能源研究所微能源系统实验室,广州,510640;中国科学技术大学热科学和能源工程系,合肥,230026
2. 中国科学院广州能源研究所微能源系统实验室,广州,510640 |
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| 摘 要： | 以微电子机械系统技术而发展起来的聚合酶链式反应(Polymerase Chain Re-action,PCR)生物芯片/微装置,由于具有所需样品和反应混合物体积少,反应速度快以及集成化程度高等优点而日益引起人们的重视。实时定量PCR是利用能特异标记PCR产物的荧光染料来动态显示PCR产物的累积,从而得到PCR扩增曲线的技术。本文介绍了实时定量检测技术及其在PCR生物芯片/微装置中的应用。
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| 关 键 词： | 聚合酶链式反应(PCR) 实时定量检测 生物芯片/微装置 荧光检测 |
| 文章编号： | 1006-6144(2006)01-0102-06 |
| 收稿时间： | 2004-04-07 |
| 修稿时间： | 2004-06-02 |
Real-time Detection in PCR Biochip/Microdevice |
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| ZHANG Chun-sun,XU Jin-liang.Real-time Detection in PCR Biochip/Microdevice[J].Journal of Analytical Science,2006,22(1):102-107. |
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| Authors: | ZHANG Chun-sun XU Jin-liang |
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| Institution: | 1. Micro Energy System Laboratory ,Guangzhou Institute of Energy Conversion, the Chinese Academy of Sciences ,Guangzhou 510640 ; 2. Department of Thermal Science and Energy Engineering ,University of Science and Technology of China, Hefei 230026 |
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| Abstract: | The PCR(polymerase chain reaction) biochips or microdevices based on microelectromechnical system technique are increasingly of interest due to the merits of small sample and reaction mixture volume,fast reaction rate and high integration and so on.Real-time PCR technique can produce and display the kinetic accumulation of PCR amplicon which is specifically labeled by the fluorescent dye-called amplification curve.In this paper,an attention is paid to introduce the applications of real-time detection technique in PCR biochips or microdevices. |
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| Keywords: | Polymerase chain reaction(PCR) Real-time detection Biochip/microdevice Application |
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