| 聚乙二醇-硫酸铵-水双水相体系萃取测定银杏叶中芦丁的含量 |
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| 引用本文： | 李蕾,范小娜,戚琦,薛君,温和瑞.聚乙二醇-硫酸铵-水双水相体系萃取测定银杏叶中芦丁的含量[J].南昌大学学报(理科版),2004,28(3):243-245. |
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| 作者姓名： | 李蕾 范小娜 戚琦 薛君 温和瑞 |
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| 作者单位： | 1. 赣南师范学院,化学与生命科学系,江西,赣州,341000
2. 赣南医学院,化学系,江西,赣州,341000 |
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| 基金项目： | 江西省自然科学基金资助项目(0320021) |
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| 摘 要： | 建立芦丁在PEG1000，PEG1500-(NH4)2SO4-H2O双水相体系中的萃取测定方法。在PEG1000，PEG1500为3．0mL，(NH4)2SO4用量为2．5g，pH6．0B．R缓冲液的最佳萃取条件下，用紫外分光光度法直接测定银杏叶提取液中的芦丁含量。实验结果表明：方法的相对标准偏差分别为2．1％，1．8％(n=7)。样品加入回收率分别为101．1％，94．7％。方法具有良好的重现性、选择性。
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| 关 键 词： | 双水相体系 萃取 紫外分光光度法 芦丁 |
| 文章编号： | 1006-0464(2004)03-0243-03 |
| 修稿时间： | 2004年2月20日 |
EXTRACTION AND DETERMINATION OF RUTIN IN GINKGO BILOBA LEAVES WITH AQUEOUS TWO-PHASE SYSTEM BASED ON POLYTHYLENE GLYEOL-(NH 4)2SO4-H2O |
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| LI Lei,FAN Xiao-na,QI Qi,XUE Jun,WEN He-rui.EXTRACTION AND DETERMINATION OF RUTIN IN GINKGO BILOBA LEAVES WITH AQUEOUS TWO-PHASE SYSTEM BASED ON POLYTHYLENE GLYEOL-(NH 4)2SO4-H2O[J].Journal of Nanchang University(Natural Science),2004,28(3):243-245. |
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| Authors: | LI Lei FAN Xiao-na QI Qi XUE Jun WEN He-rui |
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| Institution: | LI Lei~1,FAN Xiao-na~2,QI Qi~1,XUE Jun~1,WEN He-rui~1 |
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| Abstract: | The extraction method of Rutin were carried out in Aqueous Two-Phase System(ATPS) Based on polyethylene- glycol(PEG)1000,1500-(NH_4)_2SO_4-H_2O.The favorite conditions for the extraction areVPEG1000=VPEG1500=3.00mL,m(NH_4)_2SO_4=2.5g,pH=6.0 B.R.buffer.It was applied to the direct determination of Rutin in Ginkgo biloba.Leaves with Ultravioletion spectrophptometry.The experimental results showed that the Relative standard deviation of PEG1000 ATPS is 2.1%,while that of PEG1500 ATPS is (1.8%.)The recovery of sample are 101.1% and 94.7% respectively.The literature method exhibits good reproducibility and selectivity. |
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| Keywords: | aqueous two-phase system extraction ultraviolet spectrophotometry rutin |
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