呋喃唑酮在凡纳滨对虾组织中代谢动力学研究

运用液相色谱-串联质谱法研究了呋喃唑酮药饵多次给药后在凡纳滨对虾体内的药物代谢动力学.研究结果表明：呋喃唑酮代谢物在血淋巴、肝胰脏、肌肉组织中的代谢过程均符合二室模型,其动力学方程分别为C血液=414.107×e^-0.092t＋124.451×e^-0.005t,C肝胰腺=1625.563×e^-0.019t＋125.700,C肌肉=120.434×e^-0.019t＋71.579×e^-0.001t.凡纳滨对虾血淋巴和肝胰腺中呋喃唑酮代谢物浓度在4h达到高峰,肌肉中药物浓度在2h达到最大值;最高峰时血淋巴、肝胰腺和肌肉中药物浓度平均为570.6μg·L^-1、1948.6μg·kg-1和210.0μg·kg-1.在血淋巴、肝胰腺和肌肉组织中呋喃唑酮代谢物浓度的吸收半衰期（t1/2α）分别为7.497h、36.206h和36.484h,消除半衰期（t1/2β）分别为132.525 h、1 000 479.217 h和505.637 h,总表观分布容积（V1/F）分别为55.775 L·kg^-1、17.16 L·kg^-1和161.574L·kg^-1.说明给药后呋喃唑酮代谢物在凡纳滨对虾体内消除缓慢,残留严重,尤其以肝胰脏残留最为明显.

Pharmacokinetics of Furazolidone in Tissues from Litopenaeus vannamei Boone

The pharmacokinetics of furazolidone metabolite is analyzed in Litopenaeus vannamei Boone after multiple orally administration using HPLC-MS/MS method.The concentration time equations of furazolidone metabolite in blood,liver and muscle are set to be： Cblood=414.107×e^-0.092t＋124.451×e^-0.005t,Cliver pancreas= 1 625.563×e^-0.019t＋125.700,Cmuscle=120.434×e^-0.019t＋71.579×e^-0.001t,respectively.The maximum value of furazolidone metabolite of the plasma,liver and muscle is found to be 570.6 μg·L^-1,1 948.6 μg·kg^-1 and 210.0 μg·kg^-1,respectively.The absorption half-life is identified as being 7.497 h,36.206 h and 36.484 h,respectively.The elimination half life turns out to be 132.525 h,1 000 479.217 h and 505.637 h,respectively.And the apparent volume of distribution reads 55.775 L·kg^-1,17.16 L·kg^-1 and 161.574 L·kg^-1,respectively.The results show that the eliminating rate of AOZ is very low,with the residual amount in the body being significant.