Amplification immunoassay for the determination of Hepatitis B surface antigen |
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| Authors: | Atsushi Seki Eiichi Tamiya Isao Karube |
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| Institution: | Research Center for Advanced Science and Technology, University of Tokyo, Japan. |
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| Abstract: | A sensitive sandwich immunoassay for the determination of Hepatitis B surface antigen (HBs) was developed, using a cascade system of Limulus amebocyte lysate as a signal amplification system. Lipopolysaccharide (LPS) was conjugated to anti-HBs antibody. Anti-HBs antibody was adsorbed to polystyrene beads. First, HBs were reacted to solid phase anti-HBs antibody (a-HBs). After the reaction, the beads were rinsed, and were then reacted with a-HBs-LPS. Then, LPS activity specifically bound to the beads was measured. HBs could be measured in the range of 10(-10)-10(-12) g/mL. |
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| Keywords: | Index Entries" target="_blank">Index Entries Enzyme immunoassay cascade amplification hepatitis B surface antigen lipopolysaccharide limulus amebocyte lysate |
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