Microencapsulation of DNA Within alginate microspheres and crosslinked chitosan membranes for in vivo application |
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Authors: | T Alexakis D K Boadi D Quong A Groboillot I O’Neill D Poncelet R J Neufeld |
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Institution: | (1) Department of Chemical Engineering, McGill University, 3480 University St., H3A 2A7 Montreal, Quebec, Canada;(2) IARC, Lyon, France;(3) ENSAIA-INPL, Vandoeuure-les-Nancy, France |
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Abstract: | Calf thymus DNA was microencapsulated within crosslinked chitosan membranes, or immobilized within chitosan-coated alginate
microspheres. Microcapsules were prepared by interfacial polymerization of chitosan, and alginate microspheres formed by emulsification/
internal gelation. Diameters ranged from 20 to 500 Μm, depending on the formulation conditions. Encapsulated DNA was quantifiedin situ by direct spectrophotometry (260 nm) and ethidium bromide fluorimetry, and compared to DNA measurements on the fractions
following disruption and dissolution of the microspheres. Approximately 84% of the DNA was released upon core dissolution
and membrane disruption, with 12% membrane bound. The yield of encapsulation was 96%. Leakage of DNA from intact microspheres/capsules
was not observed. DNA microcapsules and microspheres were recovered intact from rat feces following gavage and gastrointestinal
transit. Higher recoveries (60%) and reduced shrinkage during transit were obtained with the alginate microspheres. DNA was
recovered and purified from the microcapsules and microspheres by chromatography and differential precipitation with ethanol.
This is the first report of microcapsules or microspheres containing biologically active material (DNA) being passed through
the gastrointestinal tract, with the potential for substantial recovery. |
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Keywords: | DNA encapsulation alginate chitosan |
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