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Effect of some parameters on the liquid chromatographic separation of biogenic amines and their metabolites |
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| Authors: | N Lammers J Zeeman G J De Jong |
| Abstract: | The liquid chromatographic separation of some biogenic amines and their metabolites with a reversed-phase ion-pair system has been optimized. The effects of the nature of the stationary phase, the column temperature, the pH, and the ion-pair reagent concentration of the mobile phase have been investigated. The most striking result is the influence of the column temperature on selectivity. |
| Keywords: | Liquid chromatography Reversed-phase ion-pair system Biogenic amines and metabolites |