Automated mass spectrometry imaging with a matrix-assisted laser desorption ionization time-of-flight instrument |
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Authors: | Markus Stoeckli Terry B Farmer Richard M Caprioli |
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Institution: | 1. Mass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
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Abstract: | The automated use of a matrix-assisted laser desorption ionization (MALDI) mass spectrometer (MS) is described for image analysis
of samples through implementation of new software for instrument control, data acquisition, and data analysis. The software
permits automated acquisition of MS MALDI spectra to form an ordered data array and contains display features to provide images
at one or more mass-to-charge ratio values. The technique can be used to scan tissue samples, blotted samples, gels, or other
sample surfaces where the image analysis of that sample is required. The program achieves a time of typically 1 s per image
point, permitting an analysis made up of large numbers of points with high spatial resolution up to 850 dpi. The features
of the software are demonstrated in this paper with samples of printed images, where visible images can be compared to those
obtained by mass spectrometry. Quantitative aspects are introduced by analyzing a series of sample spots containing different
amounts of several proteins. |
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