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Femtosecond laser-induced crystallization of protein in gel medium
Authors:Kazuhiko Nakamura  Hiroshi Y. Yoshikawa  Yoichiroh Hosokawa  Ryota Murai  Yusuke Mori  Takatomo Sasaki  Hiroshi Masuhara
Affiliation:a Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan
b Department of Applied Physics, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
c Department of Electrical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
d CREST, JST, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
Abstract:We succeeded in generating femtosecond laser-induced crystallization of hen egg-white lysozyme (HEWL) in the irradiated area by suppressing convection of the solution using a highly concentrated gel. When a laser pulse, whose energy was above the threshold energy for bubble formation, was focused along with a linear scanning of the stage, HEWL crystallization was enhanced at the surface of bubbles on the irradiated line. The relation between the bubble formation process and the crystallization is discussed.
Keywords:52.38.Mf   47.55.dd   81.10.&minus  h   87.14.Ee   87.15.Nn
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