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Three-dimensional biomolecule patterning |
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| Authors: | Maria Farsari George Filippidis Kyriaki Sambani Savas Georgiou Electra Gizeli Costas Fotakis |
| Affiliation: | a Foundation for Research and Technology Hellas, Institute of Electronic Structure and Laser (IESL), P.O. Box 527, 711 10 Heraklion, Crete, Greece b Department of Physics, University of Crete, Greece c Institute of Molecular Biology and Biotechnology (IMBB), FORTH, Greece d Department of Biology, University of Crete, Greece |
| Abstract: | A new method is demonstrated, where three-dimensional protein structures are made by employing multi-photon polymerization and photobiotin photolysis. The technique enables the construction of arbitrary two- and three-dimensional shapes with submicron resolution. The integrity of the immobilized biotin is confirmed by derivatization with fluorescently labeled streptavidin. Fluorescence microscopy is used in order to visualize the distribution of fluorescent streptavidin on the 3D structure. |
| Keywords: | Photopolymerization Biotin Nonlinear stereolithography |
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