| 黑龙江膜荚黄芪多糖及微量元素Fe、Zn、Cu和Mn的测定 |
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| 引用本文： | 李宏岩,周兆平,张莹,彭茵. 黑龙江膜荚黄芪多糖及微量元素Fe、Zn、Cu和Mn的测定[J]. 光谱实验室, 2011, 28(1): 165-168 |
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| 作者姓名： | 李宏岩 周兆平 张莹 彭茵 |
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| 作者单位： | 大连医科大学检验学院; |
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| 摘 要： | 采用水提醇沉法制备黄芪多糖提取物,苯酚-硫酸分光光度法测定多糖的含量,在0.00-0.14mg/mL线性范围内具有良好的线性关系,回收率在94.81%-103.50%.测定黄芪多糖含量为4.3%.采用校准曲线法,用空气-乙炔火焰原子吸收光谱法测定样品中Fe、Zn、Cu和Mn的含量,膜荚黄芪中微量元素含量较高.
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| 关 键 词： | 黄芪 多糖 微量元素 苯酚-硫酸分光光度法 原子吸收光谱法 |
Determination of Polysaccharide and Trace Elements Fe,Zn,Cu and Mn in Astragalus Membranaceus of Heilongjiang |
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| LI Hong-Yan,ZHOU Zhao-Ping,ZHANG Ying,PENG Yin. Determination of Polysaccharide and Trace Elements Fe,Zn,Cu and Mn in Astragalus Membranaceus of Heilongjiang[J]. Chinese Journal of Spectroscopy Laboratory, 2011, 28(1): 165-168 |
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| Authors: | LI Hong-Yan ZHOU Zhao-Ping ZHANG Ying PENG Yin |
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| Affiliation: | LI Hong-Yan ZHOU Zhao-Ping ZHANG Ying PENG Yin(College of Medical Laborotry Science,Dalian Medical University,Dalian,Liaoning 116044,P.R.China) |
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| Abstract: | The content of polysaccharides in astragalus membranaceus（fisch） was determined by phenol-sulfuric acid spectrophotometry with extracting of water and purifying of EtOH.The method had a good recovery of 94.81%—103.50% in range of 0.00—0.14mg/mL.The content of polysaccharides was 4.3%.The contents of trace elements,Fe,Zn,Cu and Mn were determined by air-acetylene flame atomic absorption spectrometry.There were the higher contents of these elements in astragalus membranaceus of HeiLongjiang. |
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| Keywords: | Astragalus Membranaceus(Fisch) Polysaccharide Trace Elements Phenol-Sulfuric Acid Spectrophotometry Atomic Absorption Spectrometry |
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