Acetylene-Substituted Two-Photon Absorbing Molecules With Rigid Elongated Pi-Conjugation: Synthesis,Spectroscopic Properties and Two-Photon Fluorescence Cell Imaging Applications |
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Authors: | Bo Liu Hai-Li Zhang Jun Liu Zhen-Li Huang Yuan-Di Zhao Qing-Ming Luo |
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Institution: | (1) Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China;(2) Hubei Bioinformatics and Molecular Imaging Key Laboratory, Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China |
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Abstract: | Two asymmetrical molecules with substituted acetylene as central rigid elongated conjugation are reported as potential chromophores
for two-photon microscopic imaging. These molecules consist of a typical D–π–A structure, have different donors (D), the same
π-conjugated center (π) and the same acceptor (A). Structural characterization and spectroscopic properties, including single-photon
(linear) absorption, quantum yields, single-photon fluorescence, and two-photon absorption spectra, were studied in solvents
with different polarity. These acetylene-substituted molecules were found to have high two-photon absorption cross-sections
(for example, 690 GM for molecule 1 in toluene), which were determined by a two-photon induced fluorescence method using a
femtosecond Ti: sapphire laser as excitation source. Single- and two-photon cellular imaging experiments demonstrate that
the substituted acetylene derivatives could be one kind of promising two-photon fluorescence probes for cellular imaging. |
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Keywords: | Spectroscopic property Structure-property relationship Substituted acetylene Two-photon absorption Two-photon microscopic imaging |
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