共振光散射法测定丁胺卡那霉素

研究了以达旦黄（TY）作为共振光散射探针测定市售药品中丁胺卡那霉素（AMK）的测定方法．该方法基于在pH=5．5的Britton—Robinson缓冲溶液中，达旦黄和丁胺卡那霉素结合后有强烈的共振光散射作用．在λ=482nm处，共振光散射强度（△IRLS）最大且光散射的强度与AMK的浓度在0．4～2．4mg·L^-1范围内成正比（相关系数r=0．9986），检出限为8．6×10^-3mg·L^-1．该方法简便、快速、灵敏，对1．0mg·L^-1的AMK溶液平行测定11次，RSD=2．57％．用于市售样品的分析测定，结果满意。

Determination of Amikin by Resonance Light Scattering Method

A new method for determination of amikin is established.The method is based on the enhancement of resonance light scattering(RLS) with the interaction of amikin and titan yellow in the Britton-Robinson buffer solution of pH=5.5.At the wavelength of 482 nm,the enhanced RLS intensity is proportional to the concentration of amikin in the range of 0.4～2.4 mg · L - 1.The detection limit is 8.6×10-3 mg · L - 1 and the relative standard deviation of amikin is 2.57 % for 1.0 mg · L-1(N=11).The proposed method is rapid,simple,sensitive and can be successfully applied for the determination of amikin in pharmaceutical formulations.