| 高效液相色谱法测定南瓜粉中的4-氨基丁酸 |
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| 引用本文： | 刘惠文.高效液相色谱法测定南瓜粉中的4-氨基丁酸[J].色谱,2001,19(6):532-533. |
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| 作者姓名： | 刘惠文 |
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| 作者单位： | 中国林业科学研究院森林生态环境研究所, |
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| 摘 要： | 采用强阳离子交换柱分离 ,pH梯度洗脱 ,邻苯二甲醛 (OPA)柱后衍生 ,荧光λex=338nm ,λem=42 5nm检测的高效液相色谱法测定了南瓜粉中的 4 氨基丁酸 (GABA)。若以GABA的峰高Y(μV)对进样质量X(μg)进行线性回归 ,则线性方程为Y =45 6 6X +1396 ,r =0 9998;GABA的平均回收率 (n =3)为 99%。方法稳定、快速、灵敏、准确。
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| 关 键 词： | 高效液相色谱法 柱后衍生 4-氨基丁酸 |
| 文章编号： | 1000-8713(2001)06-0532-02 |
| 修稿时间： | 2001年5月30日 |
Determination of 4-Aminobutyric Acid in Pumpkin Powderby High Performance Liquid Chromatography |
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| LIU Hui-wen.Determination of 4-Aminobutyric Acid in Pumpkin Powderby High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,2001,19(6):532-533. |
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| Authors: | LIU Hui-wen |
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| Institution: | Research Institute of Forest Ecology and Environmental, Chinese Academy of Forestry, Beijing 100091, China. |
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| Abstract: | A method for the separation and determination of the 4-aminobutyric acid in pumpkin powder by high performance liquid chromatography (HPLC) with post-column derivatization and fluorescence detection is described. The operating conditions were cation exchange resin column (30 cm x 0.4 cm i.d.) with gradient elution of buffer solutions A 19.6 g sodium citrate dihydrate and 1.0 g phenol dissolved in 1 L water, pH(3.15 +/- 0.02)] and B 21.0 g sodium nitrate and 1.5 g boric acid dissolved in 1 L water, pH(9.70 +/- 0.02)] as mobile phase at a flow rate of 0.5 mL/min, and a column temperature of 62 degrees C, with detection wavelength lambda ex = 338 nm, lambda em = 425 nm. The retention time of 4-aminobutyric acid was 34.77 minutes. The average recovery was 99% and the coefficient of variation was 1.35%. This method is simple, rapid and sensitive. |
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| Keywords: | high performance liquid chromatography post column derivatization 4 aminobutyric acid |
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