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Determination of mercapturic acids in urine by solid-phase extraction followed by liquid chromatography-electrospray ionization mass spectrometry
Authors:Moriwaki H  Tsujimoto Y  Noda T  Shimizu M  Tanaka M
Institution:Osaka City Institute of Public Health and Environmental Sciences, Japan.
Abstract:A novel method for the determination of five kinds of mercapturic acids, found in urine as metabolites of alkylbenzenes, based on liquid chromatography-electrospray ionization mass spectrometry is described. A solid-phase extraction procedure was used for the extraction of the mercapturic acids from urine and the separation was performed on a reversed-phase C30 column. The detection limits were in the range 2.4-3.2 ng ml-1.
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