高效毛细管电泳法同时测定减肥类功能食品中7种违禁成分

建立了同时测定减肥类功能食品中违禁成分芬氟拉明、伪麻黄碱、去甲伪麻黄碱、安非拉酮、西布曲明、西地那非和士的宁的高效毛细管电泳分析方法。以20 mmol/L硼砂-10 mmol/L SDS-5%乙腈溶液(pH9.0)为运行缓冲液,在25℃,17 kV条件下测定,检测波长为195 nm。各待测物线性范围上限均为100 mg/L,线性相关系数均大于0.998,相对标准偏差为4.5%~7.9%(n=7);平均加标回收率为79.6%~112.0%;检出限(S/N=3)为0.16~0.65 mg/L。本方法简便快速、灵敏准确、分析成本低,已成功应用于功能食品中上述减肥类违禁添加成分的测定。

Simultaneous Determination of Seven Adulterants in Functional Foods by High-Performance Capillary Electrophoresis

A high-performance capillary electrophoresis method has been established for the simultaneous determination of seven functional food adulterants, namely, fenfluramine, pseudoephedrine, norpseudoephedrine, amfepramone, sibutramine, sildenafil, and strychnine. A mixture of 20 mM sodium tetraborate-10 mM sodium dodecyl sulfate-5% acetonitrile was used as a running buffer (pH 9.0), and analysis was carried out at a detection wavelength of 195 nm under a separation voltage of 17 kV at 25 °C. The upper limit of the linear ranges was 100 mg L⁻¹, with the linear correlation coefficients being greater than 0.998. The relative standard deviations ranged between 4.5% and 7.9% (n = 7). The average recoveries were in the range of 79.6%–112.0%. The limits of detection (three times the signal-to-noise ratio, S/N = 3) were between 0.16 and 0.65 mg L⁻¹. The method is simple, fast, sensitive, accurate and cheap. It has been successfully applied for the determination of the seven target chemicals in functional foods