Estimation of cephalosporin antibiotics by differential pulse polarography

Differential pulse polarographic (DPP) method of determination of cephalosporins has been developed based on the electrochemistry of the azomethine group in the drugs in universal buffers of pH 2.0-12.0. Quantitative measurements were successful in the concentration range of 1.0 x 10(-5) M-2.5 x 10(-8) M, the lower concentration representing the detection limit by DPP. The described procedure has been applied for the determination of these drugs individually in pharmaceutical formulations and urine samples as well as for simultaneous determination in a single run.