Peroxidase-catalysed luminol chemiluminescence method for the determination of glutathione |
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Authors: | Kamidate T Watanabe H |
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Institution: | Faculty of Engineering, Hokkaido University, Kita-13, Nishi-8, Kita-ku, Sapporo, Japan. |
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Abstract: | A luminol chemiluminescence (CL) method was developed for the determination of glutathione (GSH). GSH was indirectly determined by measuring the amount of hydrogen peroxide formed during the Cu(II)-catalysed oxidation of GSH with oxygen. The amount of hydrogen peroxide formed was continuously measured using the Arthromyces ramosus peroxidase-catalysed luminol CL reaction. The CL intensities at maximum light emission were linearly correlated with the concentration of GSH over the range 7.5 x 10(-7)-3.0 x 10(-5) M. The detection limit for GSH was about 10 times better than that of the spectrophotometric method using Ellman reagent. |
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