Homogeneous liquid-liquid extraction method for spectrofluorimetric determination of chlorophyll a |
| |
| Authors: | Sudo T Igarashi S |
| |
| Affiliation: | 1. Department of Chemistry, Universidade Estadual de Maringá, Av. Colombo 5.790, 87020-900 Maringá, PR, Brazil;2. Department of Pharmacy, Universidade Estadual de Maringá, Av. Colombo 5.790, 87020-900 Maringá, PR, Brazil;1. University of Bucharest, Faculty of Chemistry, Department of Organic Chemistry, Biochemistry and Catalysis, 4-12 Regina Elisabeta Av., 030018 Bucharest, Romania;2. National Institute of Materials Physics, 405A Atomistilor Street, 077125 Magurele, Romania;3. National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, PO Box MG-36, 077125 Magurele, Romania |
| |
| Abstract: | A new homogeneous liquid-liquid extraction using a fluorocarbon ionic surfactant, Zonyl FSA (FSA), having a diethylthioether group as a spacer between the perfluoroalkyl group and carboxyl group has been developed. In this FSA method, the phase separation phenomena were observed at mild pH (below pH 6). Moreover, by using this extraction method as a preconcentration, a highly sensitive spectrofluorometric determination of chlorophyll a was established. The results for the standard chlorophyll a were as follows. The concentration factor (V(w)/V(o)) was 200 (water phase, V(w), 20 cm(3), water-immiscible phase, V(o), 100 mul), the distribution ratio, log D, was 4.85, the extraction percentage, E, was 99.7%, and the procedure time was approximately 30 min. The calibration curve was linear in the concentration range 2 x 10(-11)-3 x 10(-7) mol dm(-3) and the detection limit (S/N=3) was 1 x 10(-11) mol dm(-3). The relative standard deviation was 0.72% for 10(-8) mol dm(-3) (five determinations). |
| |
| Keywords: | |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
