光谱法研究11-羟基喜树碱与牛血清白蛋白的相互作用

在生理pH条件下用荧光光谱法和紫外光谱法结合化学计量学来研究10-羟基喜树碱(10-HCPT)与牛血清白蛋白(BSA)的相互作用,结果发现,10-HCPT对BSA的内源荧光有静态猝灭作用。以华法林作为标记药物,采用三维荧光技术,分别对竞争实验过程进行扫描,进而用平行因子法(PARAFAC)处理所得三维数据,并根据蛋白质结合位上药物的置换作用确定了10-HCPT的结合位置是BSA的site I。利用同步荧光光谱,考察了10-HCPT对BSA构象的影响,10-HCPT的加入使BSA构象发生变化,BSA内部残基所处环境的疏水性下降。

Spectroscopic studies on the interaction of 10-hydroxycamptothecin with BSA

The binding interaction of the 10-hydroxycamptothecin(10-HCPT) with bovine serum albumin(BSA),using warfarin as a fluorescence probe,was studied by spectrofluorimetry and UV-vis spectrometry with the aid of chemometrics under the simulated physiological conditions.The results indicated that the endogenetic fluorescence quenching of BSA by 10-HCPT is static quenching.In this work,the competitive interaction of the 10-HCPT and warfarin with BSA was also studied by three dimension excitation-emission fluoresce...