高效液相色谱法测定厨房用纸中双酚S

已剪碎的厨房用纸样品(0.100 0g)用10mL正己烷-四氢呋喃(1+1)混合液浸泡2h,弃去液体,用滤纸吸干厨房用纸样品表面的液体,加入10mL甲醇,在30℃超声提取30min。提取液氮吹至干,加入1mL甲醇(6+4)溶液,经0.45μm滤膜过滤,采用高效液相色谱法测定滤液中双酚S的含量。以Luna 5u C₁₈色谱柱为分离柱,以甲醇(6+4)溶液为流动相,在检测波长259nm处进行测定。双酚S的质量浓度在0.1～100.0mg·L^-1内与其对应的峰面积呈线性关系,检出限(3S/N)为0.3 mg·kg^-1。方法用于厨房用纸样品的分析,加标回收率为79.1%～104%,对双酚S标准溶液重复测定6次,测定值的相对标准偏差为0.040%～0.68%。

HPLC Determination of Bisphenol S in Kitchen Papers

The shredded kitchen paper sample(0.100 0 g) was immersed with 10 mL of the mixture of nhexane and tetrahydrofuran(1+1) for 2 h.The liquid was discarded,filter papers were used to absorb the liquid on the surface of kitchen paper sample.The dried kitchen paper sample was extracted ultrasonically for 30 min with 10 mL of methanol at 30℃.The extract was evaporated to dryness by N2-blowing,and then 1 mL of methanol(6+4) solution was added.The solution was filtered on 0.45μm filtering membrane.HPLC was applied to the determination of bisphenol S in the filtrate.Luna 5u C18 chromatographic column was used as separation column,and methanol(6+4) solution was used as mobile phase.Detection at 259 nm was adopted in the determination.Linear relationship between values of peak area and mass concentration of bisphenol S was kept in the range of 0.1-100.0 mg·L-1,with detection limit(3S/N) of 0.3 mg·kg-1.The proposed method was applied to the analysis of kitchen paper sample,giving values of recovery found by standard addition method in the range of 79.1%-104%.Bisphenol S standard solutions were tested for 6 determinations,and RSDs of determined values were in the range of 0.040%-0.68%.