Separation of peptides by HPLC using a surface-confined ionic liquid stationary phase |
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| Authors: | K R Chitta D S Van Meter A M Stalcup |
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| Institution: | (1) Department of Chemistry, P. O. Box 210172, Cincinnati, OH 45221-0172, USA;(2) Springborn Smithers Laboratories, 790 Main Street, Wareham, MA 02571, USA; |
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| Abstract: | A butylimidazolium bromide surface-confined ionic liquid stationary phase was synthesized in-house. The synthesized phase
was investigated for the separation of five peptides (Gly-Tyr, Val-Tyr-Val, leucine enkephalin, methionine enkephalin, and
angiotensin-II). The peptides were successfully separated in less than 5 min. The effect of trifluoroacetic acid (TFA) on
the separation of peptides was evaluated with results confirming that TFA was not acting as ion-pairing agent in separation
of peptides on this phase. |
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