Rapid determination of vitamin E in vegetable oils by reversed-phase high-performance liquid chromatography |
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| Authors: | Gimeno E Castellote A I Lamuela-Raventós R M de la Torre M C López-Sabater M C |
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| Institution: | Dpt. Nutrició i Bromatologia, Centre de Referència en Tecnologia dels Aliments (CeRTA), Facultat de Farmàcia, Universitat de Barcelona, Spain. |
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| Abstract: | A quick and direct method for measuring tocopherols (alpha, beta+gamma and delta) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (alpha-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a methanol-water mobile phase. Tocopherols are detected at 292 nm in less than 5 min after injection. The method is precise (RSD=2.69%) and has a high mean recovery (98.14%). |
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