Resolving domains of interdigitated phospholipid membranes with 95 GHz spin labeling EPR |
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Authors: | A I Smirnov T I Smirnova |
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Institution: | 1. Department of Chemistry, North Carolina State University, Raleigh, North Carolina, USA
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Abstract: | High-field W-band (95 GHz) electron paramagnetic resonance (EPR) study of partitioning of a small nitroxide TEMPO (2,2,6,6-tetramethyl-1-piperidinyloxy) in multilamellar liposomes composed from 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) is described. The high-resolution spectra with a high signal-to-noise ratio were combined with automated least-squares simulation analysis to derive accurate partitioning coefficients of TEMPO in the membrane lipid phase and to follow the membrane phase transitions. The isotropic magnetic parameters, giso and Aiso were used to characterize the average polarity the spin label is experiencing in the membrane. We also report an empirical correlation between giso and Aiso for a set of protic and aprotic solvents and use this correlation to assign domains formed by interdigitation of DPPC bilayer under a high ethanol concentration of 1.2 M. |
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