|
|||||
|
|
Towards a synthetic avidin mimic |
|
| Authors: | Wiklander Jesper Karlsson Björn C G Aastrup Teodor Nicholls Ian A |
| Affiliation: | 1. Bioorganic & Biophysical Chemistry Laboratory, Section for Biomaterials and Medicinal Chemistry, School of Natural Sciences, Linnaeus University, 39182, Kalmar, Sweden 2. Attana AB, 11347, Stockholm, Sweden 3. Department of Biochemistry and Organic Chemistry, Uppsala University, Box 576, 75123, Uppsala, Sweden |
| Abstract: | A series of streptavidin-mimicking molecularly imprinted polymers has been developed and evaluated for their biotin binding characteristics. A combination of molecular dynamics and NMR spectroscopy was used to examine potential polymer systems, in particular with the functional monomers methacrylic acid and 2-acrylamidopyridine. The synthesis of copolymers of ethylene dimethacrylate and one or both of these functional monomers was performed. A combination of radioligand binding studies and surface area analyses demonstrated the presence of selectivity in polymers prepared using methacrylic acid as the functional monomer. This was predicted by the molecular dynamics studies showing the power of this methodology as a prognostic tool for predicting the behavior of molecularly imprinted polymers. The biotin binding characteristics of a series of molecularly imprinted polymers have been evaluated and correlated to predictions made by molecular dynamics simulations and 1H-NMR titrations |
| Keywords: | |
| 本文献已被 PubMed SpringerLink 等数据库收录！ | |