| cDNA Cloning, Prokaryotic and Eukaryotic Expression and Characterization of Porcine Leukemia Inhibitory Factor |
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| 作者姓名： | LI Ming-tang JIANG Yong WANG Shu-mei LI Yong-ming WANG Fang HOU Xia YANG Hong MA Tong-hui |
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| 作者单位： | LI Ming-tang 1，JIANG Yong 1，WANG Shu-mei 2，LI Yong-ming 1，WANG Fang 1，HOU Xia 1，YANG Hong 1 and MA Tong-hui 1**1. Membrane Channel Research Laboratory，Northeast Normal University，Changchun 130024，P. R. China; 2. Department of Anesthesia，Second Teaching Hospital，Jilin University，Changchun 130041，P. R. China |
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| 基金项目： | 国家自然科学基金，the National Natural Science Foundation of China，吉林省杰出青年科学基金，Excellent Young Teachers Program of MOE |
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| 摘 要： | Introduction Theleukemiainhibitoryfactor(LIF)isamulti functionalcytokinebelongingtotheinterleukin6(IL6)family1].Itwasfirstfoundasamyeloidleukemic cellproliferationsuppressoranddifferentiationinducer cytokine2].ThenaturalformofLIFisa38to67kDa glycosylate…
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| 关 键 词： | 白血病抑制因子 纯系化 原核真核表达 cDNA 表征 译码 |
| 文章编号： | 1005-9040（2006）-02-145-05 |
| 收稿时间： | 2005-12-31 |
cDNA Cloning, Prokaryotic and Eukaryotic Expression and Characterization of Porcine Leukemia Inhibitory Factor |
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| LI Ming-tang JIANG Yong WANG Shu-mei LI Yong-ming WANG Fang HOU Xia YANG Hong MA Tong-hui.cDNA Cloning, Prokaryotic and Eukaryotic Expression and Characterization of Porcine Leukemia Inhibitory Factor[J].Chemical Research in Chinese University,2006,22(2):145-149. |
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| Authors: | Ming-tang LI Yong JIANG Shu-mei WANG Yong-ming LI Fang WANG Xia HOU Hong YANG Tong-hui MA |
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| Institution: | aMembrane Channel Research Laboratory, Northeast Normal University, Changchun 130024, P. R. China;bDepartment of Anesthesia, Second Teaching Hospital, Jilin University, Changchun 130041, P. R. China |
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| Abstract: | Molecular cloning of the porcine leukemia inhibitor factor(pLIF) has not been reported. A full-length cDNA encoding pLIF was cloned, expressed and characterized. The full-length porcine LIF cDNA encodes a 202 amino acid protein that has an 84% sequence identity to mouse LIF and 86% sequence identity to human LIF. The deduced amino acid sequence of a pLIF protein contains six conserved consensus N-linked glycosylation sites and six cysteine groups to form potential disulfide bonds. The pLIF was expressed in E coli, as a mature form, and in CHO cells as a secreted form. Both the forms of the recombinant pLIFs can maintain murine embryonic stem cells in an undifferentiated state in a culture. The recombinant pLIFs will be useful in establishing a long-term culture of stable pluripotent porcine embryonic stem cells for further manipulation. |
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| Keywords: | Leukemia inhibitor factor Porcine Cloning Recombinant protein Embryonic stem cell Pluripotency |
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