CIRCULAR DICHROISM OF THE PHYTOCHROME CHROMOPHORES IN NATIVE AND DENATURED STATE |
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| Authors: | Thomas Brandlmeier Harald Lehner Wolfhart Rüdiger† |
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| Institution: | Botanisches Institut der Universität München, Menzinger Strasse 67, D-8000 München 19, W. Germany;Institut für Organische Chemie der Universität Wien, Währinger Strasse 38. A-1090 Wien, Austria |
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| Abstract: | Abstract— The circular dichroism spectra of oat phytochrome were recorded. Qualitatively, the same spectra were found for large (360 kilodaltons) and small (60 kilodaltons) phytochrome. Quantitative CD data were reported for small Pr and Pfr (photoequilibrium mixture with 20% Pr) in tris buffer (native state) and in acid urea (denatured state). Further, the CD spectra of a phytochromobilinpeptide in acid solution with and without urea were recorded. Differences between the data in native and denatured state are discussed. |
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