Thin-layer chromatographic enantiomeric resolution via ligand exchange |
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Authors: | K Günther |
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Affiliation: | Fachbereich Forschung Chemie, Degussa AG, Hanau, F.R.G. |
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Abstract: | A thin-layer chromatographic technique for the separation of proteinogenic and non-proteinogenic amino acids, dipeptides and alpha-hydroxy acids is described. Other examples are given from the field of alpha-methyl, N-alkyl and halogenated amino acids. The separation of the enantiomers is achieved, without derivatization, by means of ligand exchange on a reversed-phase silica gel as stationary phase, which is covered with a chiral selector (proline derivative). The resolution is so good that the respective enantiomers can be determined at trace levels (greater than or equal to 0.25%). The proposed method is simple, inexpensive and needs no sophisticated instruments. |
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