|
|||||
|
|
High-performance liquid chromatographic analysis of Romet-30 in Chinook salmon (Oncorhynchus tshawytscha): wash-out time, tissue distribution in muscle, liver and skin, and metabolism of sulfadimethoxine |
|
| Authors: | Ming Zheng He-Yi Liu Scott F Hall David D Kitts Keith M McErlane |
| Institution: | a Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, B.C. V6T 1Z3, Canada b Department of Food Sciences, University of British Columbia, Vancouver, B.C. V6T 1W5, Canada |
| Abstract: | A high-performance liquid chromatographic (HPLC) assay was developed for the determination of Romet-30 residue levels in Chinook salmon muscle and liver tissues. The extraction recoveries averaged 66, 78 and 83% for ormetoprim (OMP), sulfadimethoxine (SDM) and N4-acetyl-SDM (N4-Ac-SDM), respectively, in muscle tissue; 61 and 72% for SDM and N4-Ac-SDM, respectively, in liver tissue. The HPLC assay had a lower detection limit of 0.05 μg/g for OMP, SDM and N4-Ac-SDM in muscle tissue, and a lower detection limit of 0.20 μg/g for SDM and N4-Ac-SDM in liver tissue. OMP could not be quantified in liver due to the presence of substantial amounts of co-extracted endogenous substances. The HPLC assay was applied to the analysis of Romet-30 residues in Chinook salmon after gastric intubation with Romet-30 at water temperatures between 8.0 and 9.0°C. Different disposition characteristics were found in muscle and liver tissues. The presence of N4-Ac-SDM in liver tissue was confirmed by MS and MS-MS analyses. An estimation of SDM and N4- Ac-SDM residue levels in skin tissue was obtained and the disposition of these two compounds was similar to the disposition in muscle tissue. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |