DNA microarray scanner with a DVD pick-up head |
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Authors: | Kyung-Ho Kim Seung-Yop Lee Sookyung Kim Seong-Gab Jeong |
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Institution: | aDepartment of Mechanical Engineering, Sogang University, Seoul 121-742, Republic of Korea;bNanostorage Co. Ltd., Seoul 120-090, Republic of Korea;cDepartment of Mechantronics, Cheongju College of Korea Polytechnic IV, Cheongju, Chungbuk 36-290, Republic of Korea |
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Abstract: | A low-cost highly sensitive DNA microarray scanner for fluorescent detection is developed based on the pick-up head of a commercially available optical storage device, DVD. A laser beam of 650 nm, generated by a DVD laser diode, is used for dynamic auto-focusing as well as the excitation of Cy5 fluorescent dye. The fluorescence intensity emitted from Cy5 dye is measured by a photomultiplier tube (PMT). In contrast to other microarray scanners, the DVD-based scanner offers the auto-focusing function using the focus error signal (FES) and a voice coil motor (VCM), and this enables fast response, high accuracy and compact size. The fluorescence-detecting performance of the scanner is inspected by using a commercial BAC (bacterial artificial chromosome) oligonucleotide chip and a scanner evaluation microarray (DS01). Experiments have shown that the DVD-based scanner meets the limit of detection, ensuring the feasibility of a low-cost, highly sensitive DNA microarray scanner. |
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Keywords: | DNA microarray DNA chip Biochip DVD Fluorescent scanner Auto-focusing actuator |
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