Effect of high pressure treatment on sarcoplasmic reticulum |
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| Authors: | Atsushi Suzuki Akira Okamoto Yoshihide Ikeuchi Makoto Saito |
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| Affiliation: | 1. Department of Applied Biochemistry, Faculty of Agriculture , 2-8050 Ikarashi, Niigata, 950-21, Japan;2. Electron Microscopic Laboratory, University of Niigata , 2-8050 Ikarashi, Niigata, 950-21, Japan |
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| Abstract: | Abstract This paper describes the changes in biochemical activities of sarcoplasmic reticulum(SR) and Ca2+ release from SR in the rabbit skeletal muscle subjected to a high pressure treatment(100∽300 MPa) to investigate the mechanism for pressure-induced meat tenderization and acceleration of meat conditioning. Pressure-dependent changes were observed in the both extra and basal ATPase activities of SR prepared from the muscles exposed to high pressure. Ca2+ uptaking ability of SR vesicles measured by fluorescent chelating reagent, indo-1, decreased with increasing pressure applied to the muscle. Ultrastructural studies revealed that Ca2+ which was mainly localized in the SR region of the untreated fiber bundles was translocated into myofibrils in the pressurized muscle. Obtained results in this report may be one of the causes for meat tenderization and acceleration of meat conditioning induced by high pressure treatment. |
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