Synthesis and secretion of human atrial natriuretic peptide in Saccharomyces cerevisiae |
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| Authors: | N Qin N Wei X H Yao Y Y Li |
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| Affiliation: | Institute of Genetics, Fudan University, Shanghai, PRC. |
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| Abstract: | A chemically synthesized alpha-hANP gene was inserted into plasmid YFD18, which was an expression-secretion vector of yeast. The recombinant then transformed in the yeast Y33. The expression level of yeast transformants was about 700 micrograms ANP/L detected by RIA. More than 99% of expression products were secreted in the culture medium. N-terminal analysis of purified product showed that the first 4 amino acid residues of alpha-hANP were deleted. |
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